Firstly, would you please try again to install the HotFix instead of disabling TCPAuto-Tuning and Windows Scaling heuristics via netsh manually.
If issue persists, please try following:
1. Test the issue in Safe Mode with Networking.
2. Disable "Large Send Offload".
How to:
1) Open an elevated command prompt and press Enter:
netsh int ip set global taskoffload=disabled.
2) Disable and re-enable the network interface or reboot your system.
3) Run the following command in an elevated command prompt to confirm the command above is successful:
netsh int ip show offload
3. Check the value of “Speed & Duplex” is Auto in Advanced Properties of the NIC. If not, please let us know your value.
4. Check if “Flow Control” is disabled in Advanced Properties of your NIC.
5. Go to your Start Menu, search for “Local Security Policy“ and run it as administrator. Under Security Settings -> Local Policies -> Security Options.
1) Open “Network security:Minimum session security for NTLM SSP (including RPC based) Clients”, unselect “Require NTLMv2 session security” and “Require 128-bit encryption”.
2) Do the same operation to “Network security:Minimum session security for NTLM SSP (including RPC based) Servers”.
3) Open the policy “Network Security LAN Manager authentication level“. Locate and select the option “Send LM & NTLM – use NTLMv2 session security if negotiated“. Click Apply, and close out of all policy management windows.